Isolation and characterization of Salmonella spp. from food and food contact surfaces in a chicken processing factory

Isolation and characterization of Salmonella spp. from food and food contact surfaces in a chicken processing factory

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ABSTRACT: The presence of Salmonella serotypes is a major safety concern of the food industry and poultry farmers.This study aimed to isolate and identify Salmonella spp.from a chicken processing facility by PCR and pulsed-field gel electrophoresis (PFGE).

In addition, the biofilm-forming abilities of the isolated bacteria on stainless steel, silicone rubber, plastic, and chicken skin were also 8SB@PK investigated.PCR was used for the confirmation of Salmonella serotypes, and then gene similarity within the same serotype was analyzed by PFGE.As a result, 26 S.

Enteritidis isolates were detected at a high rate from both food contact surfaces and chicken products during processing.All of them were 100% genetically identical to the same bacteria.The results indicated that the virulence factors and effective biofilm-forming ability of S.

Enteritidis isolates could affect human health and economic revenue.It was also suggested that the visual observation of food and food contact surfaces could be a great concern in the future.The continuous monitoring of S.

Enteritidis ORG RED QUINOA molecular and biofilm characteristics is needed to increase food safety.